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You can also concatenate a static picture to the video file. In order for this to work you need to have the same duration of both input streams though. When you are done you can multiplex the new video and new audio together by: ffmpeg -i new_video.mp4 -i new_audio.mp4 -c copy audio_and_video.mp4 The audio, on the other hand, has a normally 44100Hz or 48000Hz sampling rate, which means that the minimum sample duration with 48KHz is 2.08(3)E-5 seconds. The duration of your frame depends on the frame rate but usually is between 16,(6)ms for 60fps (1/60) content and 41,(6)ms for 24fps (1/24) You can have 1ms frame only if your video is 1000fps which I really doubt it is. Please note that 1 frame is different than 1 millisecond. ffmpeg -i input.mp4 -c:a copy -vn audio_only.mp4 The -an option is disabling the audio -c:v copy is copying your video stream to video_only.mp4. ffmpeg -i input.mp4 -c:v copy -an video_only.mp4

I am not sure I understand your question correctly but you can extract the video and audio stream, format them the way you want and then re-multiplex them all with ffmpeg.